CRISPR Cas9 Technology

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What you’ll learn:

• Learn How CRISPR-Cas9 system can modify DNA with greater precision than existing technologies.
• Used to resolve mutations and to introduce site-specific therapeutic genes in human cells so that, correcting disease-causing mutations, and alleviate disease
• Learning experience from experienced and certified Trainers.
• Hands-on Projects to practice various concepts & tools, evaluated by our lead trainer.

Description:

What is CRISPR-Cas9?

CRISPR-Cas9 is a unique technology that enables geneticists and medical researchers to edit parts of the genome by removing, adding or altering sections of the DNA sequence.

It is currently the simplest, most versatile and precise method of genetic manipulation and is therefore causing a buzz in the science world.

CRISPR-Cas9 is a genome editing tool that is creating a buzz in the science world. It is faster, cheaper and more accurate than previous techniques of editing DNA and has a wide range of potential applications.

How does it work?

1. The CRISPR-Cas9 system consists of two key molecules that introduce a change (mutation) into the DNA. These are:

⦁ an enzyme called Cas9. This acts as a pair of ‘molecular scissors’ that can cut the two strands of DNA at a specific location in the genome so that bits of DNA can then be added or removed.

⦁ a piece of RNA called guide RNA (gRNA). This consists of a small piece of pre-designed RNA sequence located within a longer RNA scaffold. The scaffold part binds to DNA and the pre-designed sequence ‘guides’ Cas9 to the right part of the genome. This makes sure that the Cas9 enzyme cuts at the right point in the genome.

2. The guide RNA is designed to find and bind to a specific sequence in the DNA. The guide RNA has RNA bases that are complementary to those of the target DNA sequence in the genome. This means that, at least in theory, the guide RNA will only bind to the target sequence and no other regions of the genome.

3. The Cas9 follows the guide RNA to the same location in the DNA sequence and makes a cut across both strands of the DNA.

4. At this stage the cell recognizes that the DNA is damaged and tries to repair it.

5. Scientists can use the DNA repair machinery to introduce changes to one or more genes in the genome of a cell of interest.

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Job Scope

Jobs directly related to your degree include:

· Academic researcher

· Biomedical scientist

· Clinical research associate

· Clinical scientist, genomics

· Clinical scientist, immunology

· Genetic counselor

· Plant breeder/geneticist

· Research scientist (life sciences)

· Research scientist (medical)

Jobs where your degree would be useful include:

· Biotechnologist

· Data scientist

· Epidemiologist

· Forensic scientist

· Medical science liaison

· Physician associate

· Scientific laboratory technician

· Science writer

· Secondary school teacher

Salary Range Provided in Genetic Engineering field:

Candidate in jobs or training period ranges from 2-2.8 Lakh per annum

For research associate, it ranges from 3.5 lakh to 5 lakh per year.

Junior scientists vary from 6-9 lakh per annum

Whereas for a senior scientist, it varies between 15-16 lakh per annum in private companies.

Not only in India but in foreign also if you are looking for a career the average annual base salary is $159,339 to $194,895 of geneticists in the USA. For example, a doctoral researcher gets a package of more than 22 lakh per annum (i.e. € 29,060/ year) whereas for postdoctoral researchers more than 50 Lakh per annum (€50,525/year). As the experience in this field increases, the salary packages offered in both private and government sector increases.

Who can enroll?

· Students pursuing their Life Science / Biotechnology / Bioscience / BSc / BTech / MSc / MTech who aspire to work in the clinical research field.

o Frequently Asked Questions:

o What is the time limit to complete the course?

Unlimited access until you decides to complete the course. But once you have given your certification test, the course ends. If you fail in the test, you can revise the course again

o How do I evaluate myself?

Time to test all the knowledge you have gained. Take the test at the end of the course and evaluate yourself. Every Learning Course has a test at the end.

o How to get the certificate?

To avail, the certificate, take the test at the end of the course. Clearing the test (with a minimum of 50%) will earn you the certificate. The certificate can be downloading from our website. The certificate has a unique code by which anybody can verify the certificate on our website.

Who this course is for:

• Students pursuing their Pharmacy / Life Science / Biotechnology / Bioscience / BSc / BTech / MSc / MTech who aspire to work in the Research Industry.

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